Figure 2
From: Water channel pore size determines exclusion properties but not solute selectivity
Relative single channel glycerol and urea permeabilities of AQP4 selectivity filter mutants. (A) Cell swelling rate constants of HEK293 cells transfected with AQP4 mutants, calculated from calcein fluorescence time-series data. (B) Cell-surface expression of AQP4 mutants in HEK293 cells measured by cell-surface biotinylation and neutravidin ELISA. (C,D) Representative calcein fluorescence timeseries from HEK293 cells transfected with AQP4 H201A or H201G and exposed to iso-osmotic solutions containing 150 mM of urea or glycerol. (E) Cell swelling rate constants from (A) above normalized to surface expression and then normalized to AQP3 glycerol permeability. This gives a relative single channel permeability in units in which AQP3 glycerol permeability is 1. (F) Normalized permeabilities for the analogous mutants of AQP1 for comparison. Panels A, B, D and F are adapted from P. Kitchen PhD thesis35.
