Figure 5 | Scientific Reports

Figure 5

From: Adipose-derived Mesenchymal Stromal Cells Modulate Lipid Metabolism and Lipid Droplet Biogenesis via AKT/mTOR –PPARγ Signalling in Macrophages

Figure 5

mTOR/PPARγ modulate lipid droplet biogenesis induced by ASCcm in macrophage. Macrophages were pre-treated with mTOR inhibitor rapamycin (20 nM) or PPARγ inhibitor GW9662 (10 nM), 30 min before treatment with ASCcm (50%) for 24 h. In (A) lipid droplets were manually enumerated by light microscopy in 50 consecutive cells or evaluated by ImageJ software analysis by the measurement of the fluorescent area. In (B) lipid droplets images were captured by fluorescent microscopy after Oil Red O staining. Analysis of PGE2 production by macrophage was performed by EIA in the supernatant. (C) Data are expressed as mean ± SEM obtained in three independent experiments. *p < 0.05 versus non treated cells (CTRL) and #p < 0.05 versus ASCcm treated cells.

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