Figure 2 | Scientific Reports

Figure 2

From: Innate immune responses through Toll-like receptor 3 require human-antigen-R-mediated Atp6v0d2 mRNA stabilization

Figure 2

Reduced response to TLR3 in HuR knockdown cells. MEF cells (a–c) or RAW264.7 cells (d–f) were infected with a retrovirus expressing scrambled or HuR-directed shRNA and were selected with puromycin. Expression of HuR in MEF cells was confirmed with western blotting (WB) (a) and RT–qPCR (b). (c) MEF cells were stimulated with poly(I:C) and the expression levels of Ifnb1 and Cxcl10 were quantified with RT–qPCR. Expression of HuR in RAW264.7 cells was confirmed with WB (d) and RT–qPCR (e). (f) RAW264.7 cells were stimulated with poly(I:C) and the expression levels of Ifnb1 and Cxcl10 were quantified with RT–qPCR. Data are the means ± SE of triplicate independent experiments. *p < 0.01, Student’s t test.

Back to article page