Figure 4
Reduced TLR3 response in ATP6V0D2 KO cells. ATP6V0D2 KO cells exogenously expressed FLAG–ATP6V0D2 after retroviral infection. After puromycin selection, Atp6v0d2 expression was detected with western blotting (WB) (a) and RT–qPCR (b). (c) Following stimulation with poly(I:C) for 8 h, Ifnb1 and Cxcl10 expression was quantified with RT–qPCR. (d) WT and ATP6V0D2 KO cells were stimulated for the indicated times, and the cell lysates were subjected to WB with an anti-pIRF3, anti-IRF3, anti-pIkBα or anti-IkBα antibody. Data are the means ± SE of triplicate independent experiments. *p < 0.01, Student’s t test.
