Table 1 Chromatographic and spectrometric characteristics of secondary metabolites identified in H. perforatum by LC(ESI-)HRMS.
No | Rt (min) | EC [M-H]− | Experimental | Theoretical | Δm ppm | RDBeq | Identification of compound |
|---|---|---|---|---|---|---|---|
[M-H]− m/z | |||||||
1 | 0.65 | C7H11O6 | 191.0544 | 191.0561 | −8.730 | 2.5 | Quinic acid |
2 | 6.14 | C16H17O9 | 353.0835 | 353.0819 | −12.363 | 8.5 | Chlorogenic acid |
3 | 7.60 | C17H19O9 | 367.0990 | 367.0976 | −12.137 | 8.5 | 3-O-Feruloylquinic acid |
4 | 8.08 | C21H19O12 | 463.0826 | 463.0823 | 0.615 | 12.5 | Hyperoside |
5 | 8.16 | C21H19O12 | 463.0828 | 463.0823 | 1.077 | 12.5 | Isoquercitrin |
6 | 8.67 | C21H19O11 | 447.0880 | 447.0874 | −12.536 | 12.5 | Isoquercetrin |
7 | 8.72 | C21H19O11 | 447.0877 | 447.0874 | −12.334 | 12.5 | Quercitrin |
8 | 10.09 | C15H9O7 | 301.0319 | 301.0295 | −11.480 | 11.5 | Quercetin |
9 | 10.36 | C27H29O16 | 609.1388 | 609.1402 | −1.965 | 13.5 | Rutin |
10 | 11.41 | C30H17O10 | 537.0762 | 537.0827 | −12.195 | 22.5 | I3,II8 Biapigenin |
11 | 12.04 | C30H17O10 | 537.0757 | 537.0827 | −12.994 | 22.5 | Amentoflavone |
12 | 14.34 | C36H27O15 | 699.1268 | 699.1355 | −12.463 | 23.5 | Skyrin-2-O-glucopyranoside |
13 | 20.37 | C30H43O4 | 467.3113 | 467.3167 | −11.564 | 9.5 | Hyperfirin |
14 | 22.20 | C35H51O4 | 535.3730 | 535.3793 | −11.649 | 10.5 | Hyperforin |
15 | 22.74 | C30H15O8 | 503.0715 | 503.0714 | −11.352 | 23.5 | Hypericin |
16 | 23.27 | C30H15O9 | 519.0661 | 519.0663 | −11.723 | 23.5 | Pseudohypericin |
