Table 1 Primers used in end-point PCR.

Ca_v3.1 external

NM_009783

F: 5′-TGT GGA AAT GGT GGT GAA GA-3′

150 bp

R: 5′-ACT GCG GAG AAG CTG ACA TT -3′

Ca_v3.1 internal

F: 5′-GAT GGT CGC TTT GGG TAT CT-3′

131 bp

R: 5′-ACT GCG GAG AAG CTG ACA TT-3′

Ca_v3.2 external

NM_021415

F: 5′- TTC ATT GTC ATG GCT GGC AT-3′

289 bp

R: 5′- TCA GGT TGT TGT TCC TGA CG-3′

Ca_v3.2 internal

NM_021415

F: 5′-TAC TCT CTG GAC GGA CAC AA-3′

262 bp

R: 5′-TCA GGT TGT TGT TCC TGA CG-3′

Ca_v3.3 external

NM_001044308

F: 5′-GAT CCT GCA GGT CTT TGA TGA-3′

239 bp

R: 5′-GAA CAC GGT TGA TGG CTT TG-3′

Ca_v3.3 internal

F: 5′-TGG GCA TTT TTG GCA AGA A-3′

129 bp

R: 5′-CAG TGC GGA TGG CTG ACA-3′

Ca_v1.2

NM_001159533

F: 5′- CAG GAG GTG ATG GAG AAG CCA -3′

315 bp

R: 5′-CTG CAG GCG GAA CCT GTT GTT-3′

Ca_v1.3 external

NM_028981

F: 5′- TGC TGT GAG GAC GAC AGC TCT CCC A -3′

342 bp

R: 5′- TAG GCC TGC AAC GGC CAT GAT CTG C -3′

Ca_v1.3 internal

F: 5′- CAG CTC CAT GGA CTT TGA GAG -3′

274 bp

R: 5′- TAG GCC TGC AAC GGC CAT GAT CTG C -3′

Smooth muscle α-actin

NM_007392

F: 5′-GTG AAG AGG AAG ACA GCA CAG-3′

146 bp

R: 5′-GCC CAT TCC AAC CAT TAC TCC-3′

eNOS

NM_008713

F: 5′-CTG CCA CCT GAT CCT AAC TTG-3′

143 bp

R: 5′-CAG CCA AAC ACC AAA GTC ATG-3′

exon1A

NM_009781

F: 5′-ATG ATT CGG GCC TTT GTT CAG CC-3′

324

R: 5′-GGA ACT GAC GGT AGA GAT GGT TGC-3′

exon1B

NM_001159533

F: 5′-ATG GTC AAT GAA AAC ACG AGG ATG-3′

234

R: 5′-GGA ACT GAC GGT AGA GAT GGT TGC-3′

exon8A

NM_009781

F: 5′-GCT GAC GGT GTT CCA GTG TA-3′

155

R: 5′-TCA AAA CAC CGA GAA CCA GA-3′

exon8B

NM_001290335

F: 5′-GCA TCA CCA ACT TCG ACA AC-3′

190

R: 5′-GCT AAG AAC ACC GAG AAC CAA-3′

exon9*-

NM_009781

F: 5′-ACC ATG GAG GGC TGG ACA GA-3′

339

exon9*+

NM_001290335

R: 5′-AGA CTC AGT CTC ACT TGT GGG-3′

414

exon31

NM_009781

F: 5′-GAG CAT AAT TGA TGT CAT TC-3′

136

R: 5′-CTT CAC CAG GCG CAT GAC-3′

exon32

NM_001255997

F: 5′-TGT TGA TAT AGC AAT CAC C-3′

130

R: 5′-CTT CAC CAG GCG CAT GAC-3′