Figure 2 | Scientific Reports

Figure 2

From: An inhibitor of apoptosis protein (EsIAP1) from Chinese mitten crab Eriocheir sinensis regulates apoptosis through inhibiting the activity of EsCaspase-3/7-1

Figure 2

The mRNA expression of EsIAP1 in crabs and subcellular localization of EsIAP1 in hemocytes. (a) Quantitative real-time PCR (qRT-PCR) analysis of the expression level of EsIAP1 mRNA in different tissues. The different letters show that there exist significant differences comparing with other groups (p < 0.05). (b) SDS-PAGE and western blotting analysis of rEsIAP1. Lane M: protein marker; Lane 1: negative control for rEsIAP1 (without IPTG induction); Lane 2: IPTG induced rEsIAP1; Lane 3: purified rEsIAP1. Lane M1: protein marker; Lane 4: western blotting analysis of the rEsIAP1; Lane 5: western blotting analysis of the pre-immune serum from mice; Lane M2: protein marker. (c) The specific antibody detection of native EsIAP1. (d) Localization of EsIAP1 in hemocytes. Immunohistochemistry was performed to analyze the expression of EsIAP1 in hemocytes of E. sinensis. After incubation of polyclonal antibody of EsIAP1 or pre-immune serum (negative control), Alexa Fluor 488-labeled goat-anti-mouse antibody was used to detect EsIAP1. Nucleus was stained with DAPI (blue). Positive signals of EsIAP1 were shown in green. Scale bar = 20 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article).

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