Figure 5 | Scientific Reports

Figure 5

From: An inhibitor of apoptosis protein (EsIAP1) from Chinese mitten crab Eriocheir sinensis regulates apoptosis through inhibiting the activity of EsCaspase-3/7-1

Figure 5

Interaction between EsIAP1 and EsCaspase-3/7-1 in vitro. (a) Purified rEsCaspase-3/7-1 (His). Lane 1: negative control for rEsCaspase-3/7-1 (His) (without IPTG induction); Lane 2: IPTG induced rEsCaspase-3/7-1 (His); Lane 3: purified rEsCaspase-3/7-1 (His). (b) Purified rEsIAP1 (GST). Lane 1: negative control for rEsIAP1 (GST, without IPTG induction); Lane 2: IPTG induced rEsIAP1 (GST); Lane 3: purified rEsIAP1 (GST). (c) Pull down by rEsIAP1 (GST). Lane 1: purified rEsIAP1 (GST); Lane 2: purified rEsCaspase-3/7-1 (His); Lane 3: washed liquid; Lane 4: eluted liquid. (d) Pull down by rEsCaspase-3/7-1 (His). Lane 1: purified rEsCaspase-3/7-1 (His); Lane 2: purified rEsIAP1 (GST); Lane 3: washed liquid; Lane 4: eluted liquid. (e) The activity of rEsIAP1 was detected with caspase-3 activity assay kit. (f) Model for EsIAP1 involvement in apoptosis pathway. A. hydrophila could activate caspase-mediated apoptosis pathway to initiate the activity of EsCaspase-3/7-1 to lead to hemocyte apoptosis. EsIAP1 could combine with EsCaspase-3/7-1 to inhibit the hemocyte apoptosis.

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