Figure 3
MCT1 (A), MCT2 (B), MCT4 (C), LDHA (D), and LDHB (E) expression measured by mRNA sequencing on human T lymphocytes in both resting and activated states obtained from 4 donors. Each color corresponds to a different donor. Only the expression of LDHA was significantly different between the two states; (F) [2,3-13C2]lactate-to-[2,3-13C2]pyruvate ratio deduced from LC-MS experiments performed on human T lymphocytes from 4 other donors following the incubation of the cells in a medium containing 5 mM sodium [2,3-13C2]pyruvate for 1 min at 37 °C. The ratio significantly increases when comparing the activated to the resting state.
