Figure 1
From: NLRP6 self-assembles into a linear molecular platform following LPS binding and ATP stimulation
Structure of NLRP6 monomer. (a) Schematic of the 2D domain structure of NLRP6. (b) Full-length human NLRP6 was purified as putatively activated oligomers. (c) Homogenous monomer preparations were obtained from NLRP6 oligomers. (d) 3D reconstruction of NLRP6 monomer, shown in three orientations. A characteristic horseshoe-like shape at the top indicates the location of LRR domain. (e) A computational model of NLRP6 monomer was built based on the closest homologous structure of NLRC4 (LRR and NOD domain: 4KXF, PDB) and PYD domain of NLRP6 (6NCV, PDB), and modelled into the 3D-EM density map. See also Supplementary Table 1, Supplementary Fig. 1.
