Table 1 Microbial components tested in ligand screening experiments.

From: NLRP6 self-assembles into a linear molecular platform following LPS binding and ATP stimulation

Microbialcomponent

Product code/company

Molar ratioa

C12-iE-DAP

tlrl-nodkit2, InvivoGen

1:20

E-DAP

tlrl-nodkit2, InvivoGen

1:20

L18-MDP

tlrl-nodkit2, InvivoGen

1:20

MDP

tlrl-nodkit2, InvivoGen

1:20

M-TriDAP

tlrl-nodkit2, InvivoGen

1:20

M-TriLYS

tlrl-nodkit2, InvivoGen

1:20

PGN-Ecndiultra pure

tlrl-nodkit2, InvivoGen

1:20

PGN-Sandi ultra pure

tlrl-nodkit2, InvivoGen

1:20

Tri-DAP

tlrl-nodkit2, InvivoGen

1:20

Murabutide

tlrl-nodkit2, InvivoGen

1:20

LPS (Ra)

L9641, Sigma

1:20

dsDNA

Syntheticb

1:20

ssDNA

Syntheticb

1:20

Natural DNA

Benzonase, Merckc

1:20

Natural RNA

Benzonase, Merckc

1:20

  1. aMolar of NLRP6/Molar of Microbial component = 1:20.
  2. b25-mers dsDNA and 25-mers ssDNA used in ligand screening experiments are synthetic.
  3. cNatural DNA and RNA used in ligand screening experiments were pre-treated with nuclease (Benzonase, Merck).
Back to article page