Table 2 Yields of Aβ40 and Aβ42 variants reported in literature and herein.
From: High-yield Production of Amyloid-β Peptide Enabled by a Customized Spider Silk Domain
Aβ variant | Fusion partner/expression method | Purified Aβ peptide yield in mg/L in rich medium | Purified Aβ peptide yield in mg/L in minimal medium | Reference |
|---|---|---|---|---|
Aβ(1–40) | NT*FlSp | 40 ± 5 | 13 ± 4 | here |
(NANP)19 | 22 | — | ||
IFABP | 4 | — | ||
GST | 7 | 1.5 | ||
Aβ(M1–40) | Directly from inclusion bodies | 10–20 | — | |
Directly from inclusion bodies | — | 10–15 | ||
Co-expression with ZAβ3 | — | 4 | ||
Aβ(1–42) | NT*FlSp | 37 ± 7 | 14 ± 2 | here |
(NANP)19 | 19 | — | ||
IFABP | 3 | — | ||
IFABP | — | 6 | ||
GST | 15 | — | ||
Ub | 4 | — | ||
Aβ(M1–42) | Directly from inclusion bodies | 8 | — | |
Co-expression with ZAβ3 | — | 3 |
