Figure 4
From: D-tyrosine adds an anti-melanogenic effect to cosmetic peptides
Terminal D-tyrosine-containing pentapeptide-18 decreases melanin synthesis in human skin. (A) Schematic of the experimental strategy. Melanoderm skin models were treated with distilled PBS (control), 5 or 10 mM C-D peptide, or 1% of Kojic acid (50 μl each) eight times over 14 days. On day 14, the samples were embedded in paraffin and sectioned at 4 μm. (B) Macroscopic views of the cultured human skin model (top panel). Skin darkness value was analyzed using the ImageJ program (bottom panel). *P < 0.05. (C) Paraffin-embedded tissue sections were either stained using hematoxylin and eosin (top panel) or Fontana-Masson (middle panel). Scale bars = 50 μm. Relative amounts of melanin in hematoxylin and eosin-stained sections were measured with the ImageJ program (bottom panel). **P < 0.01.
