Figure 7
From: Recombinant thrombomodulin prevents acute lung injury induced by renal ischemia-reperfusion injury
Histone levels in the lungs and plasma after renal IR. The effect of renal IR and rTM pretreatment on histone contents in (a) the lung and (b) plasma were evaluated at 6 h and 24 h following bilateral renal IR. (n = 6 per group) (c) Pulmonary neutrophils and histones at 6 h and 24 h after bilateral renal IR were exhibited by immunostaining of Ly6G/C (neutrophil marker) and CitH3. (d) Ly6G/C- and CitH3-positive area were also calculated at 6 h and 24 h after surgery. Scale bar, 25 µm. n = 7 per group. #P < 0.05 versus sham. *P < 0.05 between the IR and IR + rTM group. **P < 0.05 between 6 h and 24 h. Abbreviations: AU, absorbance unit; CitH3, citrullinated histone H3; IR, ischemia-reperfusion; rTM, recombinant thrombomodulin.
