Figure 2
The venom of T. nattereri Brazilian fish triggers anaphylaxis in mice dependent on mast cell derived-molecules. Female BALB/c mice immunized by the i.p. injection of 10 μg of venom adsorbed in 1.6 mg of aluminum hydroxide on day 0 were boosted 7 (10 μg), 21 and 28 (1 μg) days later. At day 35, mice were challenged by a single i.p. or i.n. injection or by multiple i.n. instillations. Mice previously shaved and scratched, with hypoallergenic dermal tapes embedded in venom solution (A). Twenty-four hours, 48 or 72 h after the last challenge, the symptoms of anaphylaxis were scored (B,D) and the levels of histamine were measured in the supernatant of peritoneal exudates (C). The results represent the mean ± SEM of 5 animals/group. The dashed line represents the detection threshold. *p < 0.05 compared with control-group; #p < 0.05 compared to intraperitoneal challenged-mice.
