Figure 3
The early-phase response is characterized by anaphylactic Abs production. After the last challenge, the levels of venom specific-IgG1 and IgG2a were determined by ELISA in plasma of BALB/c sensitized-mice (A); and the titers of anaphylactic specific VTn-IgG1 in mouse and specific VTn-IgE in rats (B) were determined by PCA (B,C). The PCA titer represents the highest dilution of pooled plasma that gave a positive reaction (diameter >5 mm). VTn was fractionated by SDS-PAGE and proteins were transferred to nitrocellulose membrane that was incubaedt with pool of plasma from allergic mice diluted 1:20 and revealed with goat anti-mouse IgG. Numbers on the left correspond to molecular mass markers (D). The results represent the mean ± SEM of 5 animals/group. The dashed line represents the detection threshold. *p < 0.05 compared with control-group.
