Figure 6
The epicutaneous exposure to the VTn triggers skin inflammation. BALB/c sensitized-mice challenged with epicutaneous exposure had the skin removed for staining with H&E or toluidine blue. Black arrows indicate leukocyte influx into the subcutaneous region of the dermis. Edema and toluidine blue-positive-mast cell (square) are observed in venom exposed mice (right) in relation to the control-mice (left). The total number of leukocytes in draining lymph nodes cell (B) and cytokine-producing CD4 T cells in the supernatant of re-stimulated cell suspension were determined by flow cytometry (C). The results represent the mean ± SEM of 5 animals/group. *p < 0.05 compared with control-group.
