Figure 5 | Scientific Reports

Figure 5

From: Chronic IL-1β-induced inflammation regulates epithelial-to-mesenchymal transition memory phenotypes via epigenetic modifications in non-small cell lung cancer

Figure 5

Dynamic epigenetic modifications of the CDH1 promoter in IL-1β-induced EMT. (A) Relative fold changes of histone modifications in different EMT stages compared to the untreated cells by ChIP-qPCR. (B) A representative picture of DNA electrophoresis showing the relative abundance of methylated (M, higher band) and unmethylated (U, lower band) DNA, examined by MSP. DNA methylation level was determined by the ratio of M band to U band in each sample. (C) SLUG level-dependent histone modifications compared to the untreated cells by ChIP-qPCR. (D) DNA methylation upon IL-1β exposure in HS and LS cells, examined by MSP. Values from ChIP-qPCR were in log scale and normalized to IgG control and Tubulin control at each time point. “-d”, days after IL-1β withdrawal; “p”, previously treated with IL-1β. See also Fig. S5.

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