Figure 4

Effects of MPO-H₂O₂-Cl⁻ system on epitope recognition of primary antibodies against matrix proteins in native HCASMC-ECM. Decellularized HCASMC-ECM was left untreated (control; 0 μM) or treated with an MPO-H₂O₂-Cl⁻ system (20 nM MPO, 5–100 μM H₂O_2, 200 mM Cl⁻). Loss of recognition of specific ECM components were detected by primary antibodies against: (A) fibronectin (pAb), (B) versican (12C5 mAb), (C) type IV collagen (pAb), and (D) the formation of HOCl-generated epitopes (mAb 2D10G9). Data are presented as means ± SEM from n = 3 independent experiments, and are expressed as a % of the control data (fibronectin, versican and type IV collagen), or the % of the maximal ELISA signal detected with mAb 2D10G9, with statistical analysis carried out using one-way ANOVA with Tukey’s post-hoc tests. * indicates statistical significance from the control (0 μM added H₂O₂) at the p < 0.05 level, and ** the p < 0.01 level.