Figure 6 | Scientific Reports

Figure 6

From: Binding of myeloperoxidase to the extracellular matrix of smooth muscle cells and subsequent matrix modification

Figure 6

Effects of MPO-H2O2-Cl− system on integrity of native Type IV collagen. Decellularized native ECM was exposed to MPO-H2O2-Cl− system (20 nM MPO, 0–100 μM H2O2, 200 mM Cl−) at 37 °C for 2 h. The presence of HOCl-induced modifications to matrix proteins were detected via double immunofluorescence staining using a collagen IV pAb and mAb 2D10G9, respectively. An Alexa Fluor 488-conjugated anti-rabbit secondary antibody (green, top row) and an Alexa Fluor 594-conjugated anti-mouse secondary antibody (red, middle row) were used to visualize matrix protein-primary antibody complexes. Merged images are shown in the bottom row. Representative images from n = 3 independent experiments are shown. Scale bars: 50 μm.

Back to article page