Figure 8 | Scientific Reports

Figure 8

From: Binding of myeloperoxidase to the extracellular matrix of smooth muscle cells and subsequent matrix modification

Figure 8

Formation of carbonyl groups on decellularized HCASMC-ECM by reagent HOCl. Decellularized HCASMC-ECM was prepared then either left untreated (control; 0 μM) or treated with reagent HOCl (0–200 μM) for 2 h at 37 °C before determination of the protein carbonyl content after derivatization with 2,4-dinitrophenylhydrazine, and subsequent recognition of the corresponding hydrazone by a commercial antibody with ABTS as the chromophore. Data from n = 3 independent experiments are expressed as mean ± SEM of the absorbance values at 405 nm. Statistical analysis was carried out using one-way ANOVA with Tukey’s post-hoc tests. ** indicates significance at the p < 0.01 level.

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