Figure 4

The effects of celastrol on L-cysteine levels and the effect of acetylcysteine on ROS, apoptosis disrupted by celastrol in NB-4 cells. (a) Acetylcysteine decreased NB-4 cells ROS induced by celastrol. Bar chart shows the statistical results from three independent experiments. Asterisks indicated statistical significance (**p < 0.01, ***p < 0.001). (b) Acetylcysteine rescued NB-4 cells from increased apoptosis induced by celastrol. Bar chart shows the statistical results from three independent experiments. Asterisks indicated statistical significance (***p < 0.001). (c) L-cysteine levels were decreased after 0.5 μM celastrol treatment in NB-4 cells. Bar chart shows the statistical results from three independent experiments. Asterisks indicated statistical significance (*p < 0.05).