Figure 3
From: Quantifying Reoxygenation in Pancreatic Cancer During Stereotactic Body Radiotherapy
Box and whisker plots showing the change in hypoxic fractions using a threshold TBR > 1.4 (a) and TBR > 2.4 (b) over the course of SBRT for the irradiated (n = 18) and control (n = 3) mice from both SGPC35 cohorts that were subject to FAZA-PET imaging. Radiation reduced hypoxia in the irradiated mice, while hypoxic fraction increased over the same time period in the control mice. (c) Change in the volume fraction vu of unperfused tissue over the course of radiotherapy for irradiated (n = 6) and control mice (n = 4) from the second cohort that were imaged during treatment with DCE-MRI. (Note that of the 4 control mice that were imaged, all 4 were subject to DCE-MRI but only 3 underwent FAZA-PET imaging). Error bars indicate standard deviations at each time points across the 6 irradiated and 4 control mice. vu decreased for the irradiated mice and increased for the control mice. (d) Voxel-scale histogram of the FAZA tissue-to-blood ratio (TBR) before and after SBRT for a representative irradiated tumour; vertical dashed lines indicate TBR values of 1.4 and 3σ (=2.42) used for hypoxic fraction quantification. FAZA uptake in an axial slice through the same tumour (blue shaded region) before (e) and after (f) SBRT. (g–i) As above for a representative non-irradiated control tumour.
