Figure 3

Seed germination assay under salt and osmotic stress. The germination rate of WT and transgenic seeds was detected on a medium supplemented with (a) 200 mM NaCl and (b) 300 mM Sorbitol for 7 d. (c) Control plate without any stress treatment was maintained for the same period of time. (d) Graphical representation of percentage seed germination after 7 d NaCl and osmotic stress treatments. H1 and H2 represent high expression lines whereas Low represents low expression line seedlings. Values are means ± SE (n = 3), and each replicate comprises 150 seeds. Different letters indicate significant difference (P ≤ 0.05) with each other.