Figure 2

Localization of CTSB between adipocytes and macrophages in obese WAT. (A) Representative images of WAT sections from 8HFD mice immunostained for PLIN1, CTSB, and F4/80 (lower panel). Representative images of WAT sections from 8HFD mice immunostained for PLIN1, rabbit-IgG, and rat-IgG (upper panel). Scale bars represent 50 μm. Arrowheads indicate CLS. (B,C) Increased crown-like structures in 18HFD mice. Representative images (B) and quantitative data (CLS per > 300 adipocytes) (C) of WAT sections in ND, 8HFD, and 18HFD mice. Arrowheads indicate CLS. Values indicate the mean ± SD. Differences between values were analysed by Student’s t-test with *P < 0.05, **P < 0.01.