Table 1 Comparison of cellular concentrations of dNTPs for K562 cells quantified by the click method and radioisotope method15.

From: Quantitation of deoxynucleoside triphosphates by click reactions

 

pmol/106 cells

Ratio of (A + T)/(C + G)

dATP

dTTP

dCTP

dGTP

K562

Click

10.9 ± 2.8

33.2 ± 4.5

6.9 ± 3.9

5.9 ± 4.0

3.4

Radioisotope

9.9 ± 1.5

27.6 ± 2.2

9.9 ± 3.3

3.1 ± 0.4

2.9

HEK-293T

Click

17.1 ± 5.9

93.6 ± 19.8

10.1 ± 2.2

11.3 ± 2.8

5.2

Radioisotope

18.6 ± 9.8

92.5 ± 14.2

9.9 ± 3.0

13.0 ± 5.8

4.9

  1. Each data point represents the mean ± SD determined from two independent isolations and analyzed at least in duplicate.
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