Table 2 Effect of three anti-cancer agents on the pools of four dNTPs in HCT116 cells.
From: Quantitation of deoxynucleoside triphosphates by click reactions
HCT116 | ||||
|---|---|---|---|---|
Treatment | pmol/106 | |||
control | Dox(1 μM) | GEM (1 μM) | 5-FdU (2 μM) | |
— | 1 hr | 8 hr | 6 hr | |
Recovery | — | 8 hr | — | — |
dATP | 2.6 ± 0.2 | 3.9 ± 0.8 | Non detectable ↓(0) | 1.9 ± 0.6 |
dTTP | 7.6 ± 3.4 | 8.1 ± 2.1 | 14.1 ± 0.9 ↑ (1.9) | Non detectable ↓(0) |
dCTP | 1.2 ± 0.9 | 2.0 ± 1.6 | 0.9 ± 0.6 | Non detectable ↓(0) |
dGTP | 7.1 ± 0.8 | 5.8 ± 1.9 | 1.8 ± 1.1 ↓ (0.3) | 3.2 ± 0.3 ↓ (0.45) |
