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Figure 1

From: Lactobacillus plantarum LMT1-48 exerts anti-obesity effect in high-fat diet-induced obese mice by regulating expression of lipogenic genes

Figure 1

Inhibitory effect of L. plantarum LMT1-48 in 3T3-L1 adipocytes. (A) 3T3-L1 cells were stimulated with MDI reagent for 48 h to induce differentiation. These cells were then treated with the L. plantarum LMT1-48 extract (final concentration of 200 µg/ml). After differentiation, 3T3-L1 cells were stained with Oil red O reagent. To quantitate the inhibitory effect of L. plantarum LMT1-48 on the differentiation, Oil Red O stained fat droplets were dissolved in isopropanol, and the amount of the dissolved stain was spectrophotometrically measured at 520 nm by an ELISA reader. (B) Meanwhile, the mRNA levels of the lipogenic genes PPARγ, C/EBPα, FAS, and FABP4 in differentiated 3T3-L1 cells were quantitated. Two independent experiments were performed with n = 5 for each experiment. Error bars represent standard error of the mean (Mean ± SEM). *P < 0.05, **P < 0.01, ***P < 0.001.

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