Figure 2 | Scientific Reports

Figure 2

From: Real-time monitoring of glutathione in living cells using genetically encoded FRET-based ratiometric nanosensor

Figure 2

Designing of FRET-based GSH indicator: (A) Genetic map of the FLIP-G construct representing the genes and arrangement of restriction sites; (B) Energy transfer illustration of the developed sensor. In unbound state (left), open form of FLIP-G causes less energy transfer (i.e. low FRET) by the excitation of CFP at 435 nm. In ligand bound form, a conformational change fetches cyan and yellow FP in close proximity, which increases energy transfer efficiency (i.e. high FRET) from CFP to YFP.

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