Figure 2

Transport of different cations assayed by the fluorophores trapped inside the proteoliposomes. Dequenching of Fluozin-1 dye fluorescence with (a) TmCorA and (b) MjCorA (added at 1 min and color-coded: black—25 μM Zn²⁺, red—25 μM Cd²⁺, blue—100 μM Ni²⁺, magenta—empty liposomes with 25 μM Zn²⁺). c Dequenching of FluoZin-3 fluorescence during transport of Mg²⁺ via red—ZntB, blue—TmCorA, black—MjCorA, magenta—empty liposomes. 100 μM Mg²⁺ was added at 1 min. (d) Incapability of these proteins to transport Al³⁺ as registered by morin dye (the same coloring as in (c), 200 μM Al³⁺ was added at 1 min). Error bars represent s.e.m. from three or more technical replicates of independent batches of proteoliposomes.