Figure 7
From: A lysosomal K+ channel regulates large particle phagocytosis by facilitating lysosome Ca2+ release
Inhibiting BK suppresses the recruitment of PI(3,5)P2 to forming phagosomes. (A,B) Selected frames from time-lapse confocal microscopy of RAW264.7 macrophages transfected with TRPML1-2N-GFP and Syt VII-mCherry that were exposed to 4.5 µm IgG-coated polystyrene beads. In response to 4.5 μm IgG-coated particles, TRPML1-2N-GFP and Syt VII-mCherry were simultaneously recruited to the forming phagosomes within 4 min after phagocytosis initiation. The center of the particle is indicated with an asterisk. (C) Paxilline (1 µM) treatment inhibited the recruitment of TRPML1-2N-GFP. (D) The overall TRPML1-2N-GFP intensity was not altered by Paxilline. These data suggest that BK regulates the recruitment of PI(3,5)P2 to forming phagosomes but not the overall PI(3,5)P2 production.
