Figure 2
From: Jedi-1 deficiency increases sensory neuron excitability through a non-cell autonomous mechanism
Peripheral glia are not altered in the absence of Jedi-1. (A) TEM images of WT and KO sciatic nerves showing the morphology of the perineurial cell layer, labeled in brackets. (B) Glutamine synthetase (GS, red) and glial fibrillary acidic protein (GFAP, green) immunostaining of spinal cord (positive control) or DRG of P1 mice from WT and KO animals. (C) Ki67 (red) and DAPI (blue) staining in adult WT or KO DRG 8–12 weeks old. Circles (left axis) are Ki67+ SGCs while squares (right axis) are Ki67+ perineurial cells. 16 animals analyzed per genotype with a minimum of 5 sections 60 microns apart analyzed per animal. No statistical difference between genotypes. (D) Quantification of laminin IF pictures such as those shown in Supplementary Fig. S2C. Thickness of the perineurial sheath measured in pixels. n = 3 animals analyzed per genotype with a minimum of 8 sections analyzed per animal at least 60 microns apart. Error bars represent SEM. No statistically significant difference between genotypes. (E) Evan’s blue dye extravasation from brain, DRG, sciatic nerve (SN), and kidney in adult WT and KO mice. Absorbance normalized to weight of tissue. No statistical difference between genotypes. All images were analyzed in ImageJ version 2.0.0-rc-69/1.52p.
