Figure 3
From: Jedi-1 deficiency increases sensory neuron excitability through a non-cell autonomous mechanism
DRG neurons develop normally in the absence of Jedi-1. (A) Example pictures of toluidine blue staining of DRG sections from adult (8–12 weeks) WT and KO mice quantified in B and C. (B) Quantification of total DRG neuron counts from serially sectioned thoracic level 13 (T13) DRGs where every 12th section was counted, summed, and total neuron counts were estimated by multiplying by 12. Analysis was performed at two different developmental ages, P0 and adult animals 8–12 weeks old. Error bars indicated SEM. No statistically significant change between genotypes at either time point. (C) Frequency distribution of soma size measured from toluidine blue staining of adult DRGs WT n = 4 animals, KO n = 3 animals with a minimum of 500 cells analyzed per animal. Small DRGs less than 25 microns in diameter are indicated by the top horizontal bar. Error bars indicated SEM. No statistical difference between genotypes in any group using 2-way ANOVA. All images were analyzed in ImageJ version 2.0.0-rc-69/1.52p.
