Table 1 Basic electrical properties and parameters of evoked action potentials from WT and Jedi-1 KO neurons. Properties of WT neurons (n = 12 cells from 4 independent preparations) or Jedi-1 KO neurons (n = 12 cells from 3 independent preparations) are shown along with results of statistical comparisons performed using Student’s t-test or Mann-Whitney test for parametric and non-parametric datasets, respectively. Bold font indicates properties that were significantly different in Jedi cells compared to wild-type (* p < 0.05, ** p < 0.01).
From: Jedi-1 deficiency increases sensory neuron excitability through a non-cell autonomous mechanism
WT (n = 12) | KO (n = 12) | Statistical comparison | |
|---|---|---|---|
Membrane capacitance (pF) [mean (SD)] | 19.9 (SD 11.1) | 18.4 (SD 11) | p = 0.75 (t test) |
Membrane resistance (MΩ) [median (1st, 3rd quartile)] | 387 (168, 612) | 576 (366, 820) | p = 0.24 (Mann-Whitney test) |
Resting membrane potential (mV) [mean (SD)] | −61.5 (SD 6.9) | −57.2 (SD 4.2) | p = 0.08 (t test) |
Rheobase (pA) [median (1st, 3rd quartile)] | 138 (46, 258)* | 60 (26, 88) | p = 0.03 (Mann-Whitney test) |
AP threshold potential (mV) [mean (SD)] | −24.5 (SD 5.8) | −20 (SD 4.7)* | p = 0.048 (t test) |
Membrane potential at peak (mV) [mean (SD)] | 51 (SD 10) | 57 (SD 5) | p = 0.065 (t test) |
Maximal rising slope (V/s) [mean (SD)] | 162 (SD 76) | 138 (SD 30) | p = 0.32 (t test) |
Membrane potential at peak of afterhyperpolarization (mV) [mean (SD)] | −73 (SD 5.4) | −77 (SD 3.5) | p = 0.056 (t test) |
Amplitude of afterhyperpolarization (change from baseline) [mean (SD)] | 11.8 (SD 8.3) | 19.9 (SD 4.3)** | p = 0.009 (t test) |
Action potential amplitude (peak to AHP) [mean (SD)] | 124 (SD 12.6) | 134 (SD 7)* | p = 0.027 (t test) |
AP duration at half max (ms) [mean (SD)] | 2.0 (SD 0.46) | 1.9 (SD 0.3) | p = 0.48 (t test) |
