Figure 6
Effect of CoCl2 or DFO treatment on breast cancer cell motility. A scratch was made through a confluent layer of (A) YS1.2 or (B) pII cells, using a p20 (for YS1.2) or p1000 (for pII) pipette tip, and the gap measured at 0 time and after 24 h of DFO or CoCl2 treatment at the concentrations indicated, as described in Methods. The % wound closure was calculated as the width of the empty space after 24 h divided by the width at 0-time x 100 subtracted from 100. Histobars represent the mean ± SEM of 9 independent determinations. Asterisks denote significant difference from the control, with (*)p > 0.05, (**)p = 0.01, and (***)p = 0.001.
