Figure 2

Determination of leucine uptake activities and their sensitivities against JPH203 in bladder cancer cells. Leucine uptake activities in T24 and 5637 cells were determined by transport assays using [¹⁴C]-leucine (1 µM) in the presence or absence of JPH203 (10 µM and 20 µM). The transport assays were conducted in the presence of Na⁺. JPH203 (10 and 20 µM) inhibited [¹⁴C] L-leucine uptake (A and B). Cell apoptosis analysis by Annexin V-FITC/PI staining and flow cytometry. The cell apoptosis rate of T24 cells was slightly increased after JPH203 treatment (10 and 20 µM) (C and D). JPH203 inhibited T24 and 5637 cell proliferation (E and F), migration (G and H), and invasion (I and J). In all panels, Cont indicates DMSO only. Data represent three independent experiments with similar results. P-values were calculated by the Mann–Whitney U-test. *P < 0.05, **P < 0.01.