Figure 3 | Scientific Reports

Figure 3

From: A misprocessed form of Apolipoprotein A-I is specifically associated with recurrent Focal Segmental Glomerulosclerosis

Figure 3

Urinary ApoA-Ib can be detected using a specific antibody against the ApoA-I propeptide sequence. Plasma and urine samples of ApoA-Ib positive FSGS relapsing patients were resolved in 7-cm 2D SDS-PAGE gels using a 4–7 Ph range and stained with colloidal coomassie (panels A,B) or transferred to PVDF membranes and probed with anti-ApoA-I (panels C,D) or custom anti-proApoA-I (panels E,F). Using the antibody against ApoA-I several forms of ApoA-I were detected in plasma (C) and in urine (D), including ApoA-I (form 0), proApoA-I (form +2) and ApoA-Ib. On the other hand, the antibody anti proApoA-I only detected proApoA-I isoforms in plasma (E) and ApoA-Ib in urine (F), but not ApoA-I.

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