Figure 1
From: p53 dynamics in single cells are temperature-sensitive
p53 dynamics in individual cells (A) Schematic overview of the reporter system used to monitor p53 dynamics. P53 fused to the yellow fluorescent protein mVenus (YFP) is stably expressed under control of the EF1A promoter. H2B is fused to mCerulean (CFP) to track nuclei in time-lapse microscopy images. (B) Time-lapse microscopy images of a selected cell irradiated with 10 Gy. The marker H2B-CFP is shown in red, p53-YFP in green. (C) Median nuclear p53-YFP levels were quantified over time for the cell shown in (B). (D) P53 levels were quantified in thousands of cells. To demonstrate the heterogeneity of the response, 20 randomly picked trajectories of p53 levels after 10 Gy ionizing radiation were visualized. (E) The p53 response on the population level at 37° (black: non-irradiated control cells; blue: cells irradiated with 10 Gy). Bold lines indicate median p53 levels, shaded areas reflect the inter-quantile range between the 0.25 and 0.75 quantile. See Table S1 for number of analysed cells.
