Figure 2
From: p53 dynamics in single cells are temperature-sensitive
p53 dynamics are temperature sensitive (A–E) Population level p53 responses of pre-equilibrated cells for the temperature range from 33 °C to 41 °C (black: non-irradiated, blue: 10 Gy IR). Bold lines indicate median p53 levels, shaded areas reflect the inter-quantile ranges from 0.25 to 0.75. At 41 °C, p53 dynamics change dramatically, as pulsatile p53 accumulation gets supplanted by a strong sustained response. See Table S1 for number of analysed cells. (F–G) Cell proliferation shown as number of cell divisions per cell within 24 h for non-irradiated (F) and irradiated (G) cells incubated at the indicated temperatures. (H–I) Coefficients of variation over time for control (H) or damaged (I) cell populations shown in (A–E). At 41 °C, we detected strong increases in heterogeneity for both conditions. At other temperatures heterogeneity was mainly caused by increasing asynchrony of p53 pulses within a population. (J) p53 response of control and irradiated cell populations acutely shifted to 41 °C without prior pre-equilibration. The responses differ in amplitude and dynamics from our observations in (E). See Table S1 for number of analysed cells. (K) Accumulated p53 levels for non-irradiated and irradiated cells over the temperature range from 33 °C to 41 °C for the observation period of 24 h (left). Estimated changes compared to cells incubated at 37 °C are indicated on the right (red dots); error bars represent 95% confidence intervals determined by permutation testing. Dashed lines serve as guides to the eye.
