Figure 3
From: p53 dynamics in single cells are temperature-sensitive
Quantification of dynamic features of the p53 response (A) Quantification of the first 3 pitch positions estimated by the average magnitude difference function for irradiated cells (10 Gy) over a temperature range from 33 °C to 37 °C. Red lines indicate medians of distributions; boxes include data between the 25th and 75th percentiles; whiskers extend to maximum values within 1.5 × the interquartile range. Estimated changes compared to cells incubated at 37 °C are indicated below (red dots); error bars represent 95% confidence intervals determined by permutation testing. Dashed lines serve as guides to the eye. (B) Quantification of the inter-pitch intervals for irradiated cells (10 Gy) over a temperature range from 33 °C to 37 °C. With lower temperatures the length of the interval increases while the range of the distribution is reduced. Estimated changes compared to cells incubated at 37 °C are indicated below (red dots); error bars represent 95% confidence intervals determined by permutation testing. Dashed lines serve as guides to the eye. (C) Auto-correlation among irradiated cells at 37 °C and 41 °C. Each point in this graph represents the correlation coefficient between p53 levels in the population at two different time points, except along the main-diagonal. We detected repetitive pulses for 37 °C, but only one long homogenous response for 41 °C. (D) Probabilities of being in a certain pulse state at a given time point for irradiated cells (10 Gy) over a temperature range from 33 °C to 41 °C. Pulses are detected using a local dynamic time warping based approach. The pulsing frequency is positively correlated with the temperature within the range between 33 °C to 39 °C. At 41 °C the pulsing dynamics is completely lost and replaced by a sustained response. (E–F) Quantification the duration (E) and amplitude (F) of the first 3 pulses with respect to temperature. Estimated changes compared to cells incubated at 37 °C are indicated below (red dots); error bars represent 95% confidence intervals determined by permutation testing. Dashed lines serve as guides to the eye.
