Figure 4
From: p53 dynamics in single cells are temperature-sensitive
An ongoing p53 response is altered by changes in temperature (A) Temperature change alters the dynamics of an ongoing p53 response. Cells were incubated at 37 °C and exposed to genotoxic stress (10 Gy IR). 12 h after damage induction, approximately after the 3rd pulse of the expected canonical response, we altered the temperature in the range from 33 °C to 41 °C and followed cells for an additional 36 h. Median p53 levels of cell populations are indicated. See Table S1 for number of analysed cells. (B) Probabilities of being in a certain pulse after temperature change at 12 h.
