Table 1 Summary of the aims of this study, and the methods and genotypes used in the experiments.
Aim | Method | Genotype |
|---|---|---|
Assessment of infection responses to natural stripe rust infections under field conditions (2014–2016), in order to compare the resistance of the lines carrying 1RS of different origin. | Visual observation and scoring according to the modified Cobb scale (percentages of infected leaf area) | Mv9kr1 (parent of ‘179’), ‘Kriszta’ (parent of ‘179’), ‘179’, ‘C5’, ‘D5’ (T1BL.1RS wheat lines), DA1R (Mv9kr1/‘Kriszta’ 1R addition line), ‘Mv Magdaléna’ (T1BL.1RS - ‘Petkus’) |
Comparision of infection responses to leaf rust and powdery mildew under greenhouse conditions. | Artificial inoculation | Mv9kr1, ‘Kriszta’, ‘179’, ‘Mv Magdaléna’ |
Determination of morphological changes caused by the presence of ‘Kriszta’ 1RS (and/or lack of 1BS) in the Mv9kr1 genetic background. | Measurements both in field and after harvest | Mv9kr1, ‘Kriszta’, ‘179’ |
Detection of putative cytomolecular differences between 1RS chromosme arm of the stripe rust-susceptible ‘Mv Magdaléna’ (‘Petkus’ origin) and that of the resistant wheat line ‘179’. | Fluorescent in situ hybridization with repetitive DNA probe pSc179.2 | Várda (S. cereale, parent of ‘Kriszta’), S. strictum (parent of ‘Kriszta’), ‘R797’, ‘Kriszta’ (S. cereanum parent of ‘179’), ‘179’, ‘Mv Magdaléna’ |
Detection of changes in the storage protein composition and dietary fiber content of the line ‘179’ caused by the presence of ‘Kriszta’ 1RS arm in the Mv9kr1 genetic background. | Kjeldahl-method SE-HPLC RP-HPLC Colorimetry | Mv9kr1, ‘Kriszta’, ‘179’ |
Detection of putative differences in the ω-secalin subunit composition encoded by the Sec-1 locus on 1RS arms of different origin (‘Petkus’ vs. ‘Kriszta’). | Acid-polyacrylamid gel electrophoresis | Mv9kr1, ‘Kriszta’, ‘179’, ‘Mv Magdaléna’, Chinese Spring (as a reference) |
Detection of putative differences at the DNA level between 1RS arms of ‘Petkus’ and ‘Kriszta’ origin, and selection of ‘Kriszta’ 1RS-specific markers for future use in development of other 1RS introgression lines. | SSR and ISBP marker analyses | Mv9kr1, ‘Kriszta’, ‘179’, ‘Mv Magdaléna’ |
Assessment of allelic variations between stripe rust-resistant and -sensitive T1RS.1BL lines. | DArTseq platform | Mv9kr1 (susceptible), ‘179’, ‘C5’, ‘D5’ (resistant), ‘Mv Magdaléna’ (susceptible), DA1R (susceptible) |
