Figure 4 | Scientific Reports

Figure 4

From: Co-existence of PrPD types 1 and 2 in sporadic Creutzfeldt-Jakob disease of the VV subgroup: phenotypic and prion protein characteristics

Figure 4

Conformational solubility and stability assay (CSSA). GdnHCl1/2 quantity of GdnHCl required to solubilize half of totPrPD, (A,D), or of resPrPD, (B,C,E). (A,B) GdnHCl1/2 indexes were qualitatively similar for totPrPD (A) and resPrPD (B). In -VV1, T121 was significantly less stable than T120 while in -VV1–2 cases harboring T2 in significant amounts, T121 showed a GdnHCl1/2 similar to that of T120, both mimicking the GdnHCl1/2 of T2. (C) GdnHCl1/2 index obtained after probing resPrPD with the type-specific Abs 12B2 to T1 and To-2 to T2 were comparable with those generated using the type-generic Ab 3F4 in A and B. (D,E): Representative WB of totPrPD from -VV1 (D), and resPrPD from each sCJDVV subtype (E). *P < 0.05–0.04, **P < 0.02, ****P < 0.0006–0.0001. sCJDVV1 with T120 (N = 3) or T121 (N = 3); -VV1–2 with T120 (N = 3) or T121 (N = 4), -VV2 (N = 3).

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