Figure 2 | Scientific Reports

Figure 2

From: Evaluation of the antiviral activity of orlistat (tetrahydrolipstatin) against dengue virus, Japanese encephalitis virus, Zika virus and chikungunya virus

Figure 2

Effect of orlistat treatment on infection. HEK293T/17 cells were treated with either DMSO vehicle control or 100 μM orlistat for (A,B) 1 h pre-infection only, (C,D) post-infection only, or (E,F) pre- and post-infection and were infected at MOI of 5 with (A,C and E) nine DENVs representing the four serotypes of DENV (DENV 1DHF, DENV 1/1666, DENV 2LAB, DENV 2DF, DENV 3DF, DENV 4LAB, DENV 4DF, DENV 4DHF, DENV 4/163), and (B,D and F) two isolates of ZIKV (SV0010/15 and MR766), one isolate of JEV (BJ1), and two isolates of CHIKV (ECSA 226 V and ECSA A226). At 36 h.p.i. the percentage infection was determined by flow cytometry. Experiments were undertaken independently in triplicate. Bar graphs show mean +/− SD (*p value < 0.05, **p value < 0.01, and ***p value < 0.001).

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