Figure 1

Nanoparticle Tracking Analysis (NTA) of human plasma EVs. Quantitation (A,C) and size (B,D) of EVs isolated from either healthy controls (HC) or ME/CFS patients, using a protocol without (A,B) or with (C,D) proteinase K treatment (w/prot K). Analysis was performed with a NanoSight NS300, Malvern Panalytical. Means are shown by horizontal bars, whiskers indicate standard deviations (SD).