Figure 5

All4981 forms cell-traversing filament-like structures in cyanobacteria. (A,B) GFP fluorescence and merged GFP fluorescence and chlorophyll autofluorescence (red) and bright field micrographs of (A) Anabaena and (B) Synechocystis cells expressing All4981-GFP. Anabaena cells were grown in BG11₀ and Synechocystis cells were grown in BG11. (A) Maximum intensity projections of a Z-stack. White triangles indicate selected filament-like strings traversing through the cells. White arrows point to spindle-like All4981-GFP structures. White stars mark septal formations between two neighboring cells. Scale bars: 5 µm. (C) Excerpt of interacting proteins of interest from mass spectrometry analysis of anti-GFP co-immunoprecipitations of Anabaena cells expressing YFP-All4981^ΔTPR3–4 from P_petE.