Figure 5

LDLR activity of wt and p.(Asp492Asn), p.(Arg633Cys), and p.(Asp700Gly) LDLR variants. (A) LDL- LDLR binding, (B) FITC-LDL uptake activity and (C) LDL binding at different pH. Assays were performed as described in Materials and Methods. Data show the mean of three independent experiments; error bars represent ± SD. *P < 0.001 compared to wt using a Student’s t-test.