Figure 5

Effects of GSJD on GBM thickness, foot process form and podocin and nephrin protein expression in the kidneys of rats. (a) Representative ultrastructural changes in different groups (Scale bars 5 μm; red arrow: normal foot process, blue arrow: thickened GBM and effacement of foot process, pink arrow: fusion of foot process); (b) Quantification of GBM thickness; (c) Characteristics of podocin and nephrin protein expression in each group; (d,e) Quantitative analysis of podocin and nephrin protein expression in each group. Data were analysed by one-way ANOVA and presented as the mean ± SEM (n = 3). *P < 0.05 DN vs. NC, **P < 0.01 DN vs. NC, ^#P < 0.05 vs. DN, and ^##P < 0.01 vs. DN.