Figure 3

Dependence of holographically measured diameter, d_p, and refractive index, n_p, on particle position, z_p, within the sample cell for (a) biotinylated spheres before (yellow squares) and after (red circles) binding by NeutrAvidin and (b) spheres coated with Protein A before and after binding by IgG. Population averages and standard deviations are calculated at each height using kernel density estimators.