Figure 4
From: Context-Dependent Role of Vinculin in Neutrophil Adhesion, Motility and Trafficking
Vinculin is required for stable F-actin localization within uropods and neutrophil polarization. (a) Representative immunoflluorescence images of neutrophils after 30-minute incubation on immobilized ICAM-1 and CXCL1. Cells were stained with Hoescht, phalloidin, and anti-CD11a (n = 3 independent experiments). Scale bar = 10 µm. (b) Polarization of neutrophils based on asymmetric F-actin distribution (n = 3 independent experiments). (c,d) Background-subtracted and normalized TIRFM fluorescent intensities of F-actin and CD11a (n > 30 cells/group, 3 independent experiments). Analyzed using Kruskal-Wallis one-way ANOVA with Dunn’s multiple comparison test. ****p < 0.0001.
